Technoclone Anti-tPA Monoclonal Antibody 7VPA
IgG1; half maximal binding to coated t-PA at 19 µg/ml (low affinity); Reaction with an epitope expressed on kringle2. Competitive inhibition of plasminogen activation in the presence of fibrin (Ki=0.57 nM). It can be used for biochemical studies.
Binds to t-PA. Reaction with an epitope expressed on Kringle 2. Competitive inhibition of plasminogen activation in the presence of fibrin (Ki = 0.57nM). Half maximal binding to coated t-PA at 19.0 µg/ml (low affinity).
Hybridization of NSO cells with spleen cells from a BALB/c mouse immunized with purified melanoma-type t-PA.
The antibody is purified from murine ascites by affinity chromatography on immobilized recombinant Protein A. The purity of the product is tested by Laemmli SDS-PAGE.
The antibody is lyophilized from a 1 mg/ml solution in isotonic phosphate buffered saline, pH 7.4, containing 0.02% sodium azide and 20 mg/ml mannitol. It is supplied in vials of 500 µg and should be reconstituted with 0.5 ml distilled water.
For extensive dilutions a protein containing solution should be used (e.g. 1% bovine serum albumin in PBS).
Lyophilized antibody should be stored at 4°C. Reconstituted antibody should be aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.