Technoclone Anti-PAI-1 Polyclonal Antibody, 5mg

Reaction with PAI-1 from endothelial cells, platelets and human plasma as well as with PAI-1 from conditioned media of the human cell lines MJZJ and HT1080, recognizes free and complexed PAI-1 as well as latent PAI-1. Can be used as an antibody in an ELISA for the determination of PAI antigen and for immunoblotting experiments.

Immunizing antigen

Human recombinant PAI-1.

Immunized species

Rabbit Purification The antibody is purified from rabbit serum by ammonium sulphate precipitation followed by DEAE ion-exchange chromatography.

Characteristics of the antibody

Reacts with PAI from endothelial cells, platelets and human plasma as well as with PAI from conditioned media of the human cell lines MJZJ and HT 1080; recognises free and complexed PAI as well as latent PAI.

 

The antibody is lyophilized from a 5 mg/ml solution in isotonic phosphate buffered saline, pH 7.4, containing 0.02% sodium azide and 20 mg/mL mannitol. It is supplied in vials of 1mg and should be reconstituted with 5 ml distilled water. For extensive dilutions a protein containing solution should be used (e.g. 1% bovine serum albumin in PBS). Lyophilized antibody should be stored at 4°C. Reconstituted antibody should be aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.