This test requires professional lab infrastructure and staff and must be ordered and administered by medical professionals. The test cannot be performed by private individuals and we do not sell the kits to such subjects. If you suspect you are infected with SARS-CoV-2, please contact your healthcare provider.
SARS-CoV-2 S1RBD IgG ELISA Kit is a highly sensitive and specific immunoassay developed by ImmunoDiagnostics for the precision detection and quantitative measurement of IgG class antibodies against the S1RBD of SARS-CoV-2 virus in human blood samples. This product is for use by professional persons only.
ImmunoDiagnostics SARS-CoV-2 S1RBD IgG ELISA kit is a two-step incubation immunoassay kit. Recombinant spike protein S1 receptor binding domain (S1RBD) of SARS-CoV-2 pre-coated onto the polystyrene microwell strips can specifically recognize anti-S1RBD antibodies in human serum or plasma specimen. After a 1-hour incubation, anti-S1RBD antibodies are captured by immobilized S1RBD protein while the unbound components are washed away. Afterwards, a detection solution containing HRP-conjugated anti-human IgG is added for another 1-hour incubation, wherein HRP-conjugated anti-human IgG binds to the IgG class antibodies previously bound to S1RBD protein on the plate. After removal of nonspecific bindings, a HRP substrate solution containing 3,3′,5,5′-Tetramethylbenzidine (TMB) is added, resulting in the formation of a blue color. Color reaction is stopped by 2M H2SO4, transforming the blue color to yellow signals, which is quantified by an absorbance microplate reader at 450nm. The color intensity is proportional to the amount of anti-S1RBP antibodies captured inside the wells. The unknown sample concentration can be interpolated from a standard curve generated by the humanized anti-S1RBD monoclonal antibody (mAb).
Assay Buffer 1 x 20 ml
Wash Buffer 1 x 40 ml
Detection Antibody Solution 1 x 0.12 ml
Substrate Solution: 1 x 12ml
Stop Solution: 1 x 12 ml
Standard (Humanized Antibody S1RBI antibody) 1 x 50 ul
Results in under 2.5 hours.
Calibrated with WHO International standard reference serum to ensure accurate quantification.
Widely used for evaluation of COVID-19 vaccine effectiveness.