This test requires professional lab infrastructure and staff and must be ordered and administered by medical professionals. The test cannot be performed by private individuals and we do not sell the kits to such subjects. If you suspect you are infected with SARS-CoV-2, please contact your healthcare provider.

For quantitative determination of human anti-SARS-CoV-2 S1 protein ELISA (IgM class antibodies) in serum or plasma samples.

SARS-CoV-2 S1 IgM ELISA Kit is a highly sensitive and specific immunoassay developed by ImmunoDiagnostics for quantitative detection of IgM class antibodies against the S1 of SARSCoV-2 in human blood.

96-well plates are coated with SARS-CoV-2 S1 protein that captures antibodies against SARS-CoV2 S1 protein in the sample. After washing away unbound materials, captured IgM against SARS-CoV2 S1 protein is detected by anti-human IgM polyclonal antibodies conjugated with horse radish peroxidase (HRP). After washing step, the chromogenic substrate 3,3’,5,5’-tetramethylbenzidine (TMB) is added. Color reaction is stopped by 2M H2SO4. The amount of IgM class antibodies against SARS-CoV-2 S1 captured inside the wells is proportional to the color density generated in the coupled oxidation-reduction reaction.

Each kit is sufficient for 96 tests and contains the following components:

One aluminum pouch with a Microwell plate (12 strips of 8 wells each) coated with SARS-CoV2 S1 protein, sealed. The microwell strips can be used separately.

10 × Wash buffer- 1x 40 ml

5 × Assay buffer-   1x 20 ml

100 × Detection antibody solution: HRP-conjugated anti-human IgM, 1 x 0.12 ml

10 × Standard: Anti-S1 antibody (IgM) 1 x 50 ul

Substrate solution, 1 x 12 ml, ready for use

Stop solution, 1 x 12 ml, ready for use

Results in under 2.5 hours

Unique quantitative assay using human anti-SARS-CoV-2 S1 IgM as a standard control

 

 

High sensitivity, reproducibility, and stability