This test requires professional lab infrastructure and staff and must be ordered and administered by medical professionals. The test cannot be performed by private individuals and we do not sell the kits to such subjects. If you suspect you are infected with SARS-CoV-2, please contact your healthcare provider.
For qualitative determination of human anti-SARS-CoV-2 nucleocapsid protein (NP) ELISA (IgA class antibodies) in serum or plasma samples.
SARS-CoV-2 NP IgA ELISA Kit is a highly sensitive and specific immunoassay developed by ImmunoDiagnostics for qualitative detection of IgA class antibodies against the NP of SARSCoV-2 in human blood.
96-well plates are coated with SARS-CoV-2 NP protein that captures antibodies against SARSCoV-2 NP protein in the sample. After washing away unbound materials, captured IgA against SARS-CoV-2 NP is detected by anti-human IgA monoclonal antibodies conjugated with horse radish peroxidase (HRP), which is specific to human IgA, with no cross-reaction with human IgG or IgM. After washing step, the chromogenic substrate 3,3’,5,5’-tetramethylbenzidine (TMB) is added. Color reaction is stopped by 2M H2SO4. The amount of IgA class antibodies against SARSCoV-2 NP captured inside the wells is proportional to the color density generated in the coupled oxidation-reduction reaction.
Each kit is sufficient for 96 tests and contains the following components:
One aluminum pouch with a Microwell plate (12 strips of 8 wells each) coated with SARSCoV-2 NP protein, sealed. The microwell strips can be used separately.
10 × Wash buffer, 1 x 40 ml
5 × Assay buffer, 1 x 20 ml
100 × Detection antibody solution: HRP-conjugated anti-human IgA monoclonal antibody, 1 x 0.12 ml
10 × Human anti-NP IgA antibody (positive control), 1 x 22 ul
Substrate solution, 12 ml, ready for use
Stop solution, 12 ml, ready for use
Blank control, 0.5 ml, ready to use
Results in under 2.5 hours
Unique assay to determine anti-SARS-CoV-2 NP IgA
High sensitivity, specificity, and stability