This test requires professional lab infrastructure and staff and must be ordered and administered by medical professionals. The test cannot be performed by private individuals and we do not sell the kits to such subjects. If you suspect you are infected with SARS-CoV-2, please contact your healthcare provider.
For the qualitative determination of human anti-SARS-CoV-2 nucleocapsid protein (NP) ELISA (total antibodies) in human serum or plasma.
SARS-CoV-2 NP Ab ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the detection and qualitative measurement of total antibodies against the nucleocapsid protein (NP) of SARS-CoV-2 virus in human blood.
ImmunoDiagnostics SARS-CoV-2 NP Ab ELISA kit is a two-step incubation immunoassay kit. Recombinant nucleocapsid protein (NP) of SARS-CoV-2 pre-coated onto the polystyrene microwell strips can specifically recognize anti-NP antibodies in human serum or plasma specimens. After a 1-hour incubation, anti-NP antibodies are captured by immobilized NP protein while the unbound components are washed away. Afterwards, a detection solution containing HRP-conjugated NP is added for another 1-hour incubation, wherein HRP-conjugated NP binds to the total antibodies previously bound to NP protein on the plate. After removal of nonspecific bindings, a HRP substrate solution containing 3,3′,5,5′-Tetramethylbenzidine (TMB) is added, resulting in the formation of a blue color. Color reaction is stopped by 2M H2SO4, transforming the blue color to yellow signals, which is quantified by an absorbance microplate reader at 450nm. The color intensity is proportional to the amount of anti-NP total antibodies captured inside the wells.
1 x Microtiter plate (12 breakable strips per 8 wells)
1 x Assay Buffer (20 ml)
1 x Wash Buffer (40 ml)
1 x Detection Antibody Solution (0.12 ml)
1 x Substrate Solution (12 ml)
1 x Stop Solution (12 ml)
1 x Blank Control (1 ml)
1 x Positive Control (1 vial)
Results in under 2.5 hours
93% Sensitivity, 95% Specificity