This test requires professional lab infrastructure and staff and must be ordered and administered by medical professionals. The test cannot be performed by private individuals and we do not sell the kits to such subjects. If you suspect you are infected with SARS-CoV-2, please contact your healthcare provider.
GA CoV-2 IgG is used for the determination of IgG antibodies to SARS-Coronavirus-2.
Antibodies of the controls and diluted subject samples react with antigens immobilized on the solid phase of microtiter plates. Use of recombinant antigens guarantees the specific binding of antibodies of the specimen under investigation. Following an incubation period of 45 min at 37°C, unbound sample components are removed by a wash step.
The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 45 min at 37°C, excessive conjugate is separated from the solid-phase immune complexes by the following wash step.
HRP converts the added colorless substrate solution of 3,3’,5,5’-tetramethylbenzidine (TMB) into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature (18-25°C) turning the solution from blue to yellow.
The optical density (OD) of the solution measured at 450 nm is directly proportional to the number of specific antibodies bound.
1 x Microtiter plate (12 breakable strips per 8 wells)
1 x Conjugated wash buffer (60 ml)
1 x Sample diluent (50ml)
1 x Start reagent (8 ml)
1 x Conjugate (15ml)
1 x Substrate (15 ml)
1 x Stop solution (15ml)
1 x Positive Control (2ml)
1 x Negative Control (2ml)
Results in under 2 hours
98% Sensitivity, > 98% Specificity