Generic Assays Anti-ASGPR is a chromogenic ELISA kit for the semi-quantitative determination of IgG antibodies against asiaglycoprotein receptor (ASGPR) in human serum or plasma. ASGPR is a liver specific membrane receptor that plays a pivotal role in the endocytosis of glycoproteins from the blood stream. The induction of immune mechanisms targeting ASGPR has been observed in inflammatory liver disorders, especially auto-immune hepatitis.
Reagents, composition, storage, and stability
- Microtiter plate. 12 breakable strips with 8 wells each (96 well total) coated with rabbit ASGPR. The plate is supplied vacuum sealed with a drying agent
- Concentrated wash buffer. 100 mL of 10X concentrate wash buffer
- Sample Diluent. 100 mL of diluent. Ready to use.
- Conjugate. 15 mL of goat anti-human IgG coupled to HRP. Ready to use.
- Substrate. 15 mL of TMB in citrate buffer containing H2O2. Ready to use
- Stop Solution. 15 mL of 0.25 M sulfuric acid. Ready to use
- Positive Control. 1 mL of pre-diluted serum. Ready to use
- Cut-off Control. 1 mL of pre-diluted serum. Ready to use
- Negative Control. 1 mL of pre-diluted serum. Ready to use
Asialoglycoprotein receptor (ASGPR) is a C-type lectin that is principally expressed on the sinusoidal surface of hepatocytes. The major role of ASGPR is to clear from circulation glycoproteins containing terminal N-acetylgalactosamine or galactose via binding and cellular internalization. It has also been shown to be involved in the clearance of IgA, apoptotic bodies, low-density lipoproteins, and cellular fibronectin.
In normal hepatocytes, ASGPR is expressed in a polar manner. During liver inflammation, expression shift from the sinusoidal and basolateral surface towards the canalicular membrane. ASGPR is overexpressed in cirrhosis and end-stage liver diseases. ASGPR can frequently be the autoimmune target in immune-mediated liver diseases.
One of the major challenges in developing a molecular assay for ASGPR has been access to highly purified and immunogenic protein. Recombinant antigen has been produced, though its immunogenicity appears to be poor, according to a report by Treichel. Therefore, ASGPR obtained via chromatographic separation on galactose-sepharose, seems the best source of antigen.