Senova Von Willebrand Factor Propeptide VWF:PP
Posted: June 26, 2019
Product |
Quick and simple |
The VWF:PP double monoclonal ELISA allows a rapid determination of VWF with standard colorimetric ELISA technique:
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Microtiter strip with capture antibody 50 μl sample + 50 μl antibody-POD conjugate 60 min, 37 °C 4x washing + 100 μl substrate (TMB) 15 min + 100 μl stop solution OD reading (620/450 nm) |
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Background |
Research Applications in VWD |
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Von Willebrand Factor (VWF) is large multimeric plasma protein with important functions in primary hemostasis. It is also a carrier protein for FVIII. VWF is synthesized as a precursor. After several posttranslational modifications and cleavage of the signal peptide in the trans-Golgi-system, the protease furin cleaves the propeptide of VWF (VWF:PP) that remains associated to VWF in Weibel-Palade bodies in endothelial cells or in α-granules of megakaryocytes. Stimulation of these cells releases the complex into plasma, where both components dissociate and get metabolized with a different half live:
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The determination of VWF:PP is an important step for researching VWF deficiency (VWD). The ratio of VWF:PP and VWF:AG indicates the clearance of VWF. An elevated ratio is typically found in VWD The VWF:PP / VWF:Ag ratio can be used to study the differentiation of VWD types. VWF:PP could be measured to study acquired VWD in research fields like ECMO (extracorporeal membrane oxygenation) test plasma, cardiac disorders (e.g. aortic stenosis, congenital cardiac defects, mitral valve prolapse), in hematoproliferative diseases, monoclonal gammopathy, myeloma, other lymphoproliferative disorders, immunologic diseases, (e.g. SLE); thyroid disorders, diabetes, nephropathies, DIC, sepsis and other diseases. RESEARCH USE ONLY IN U.S. & CANADA |
Order info |
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| Diapharma Group, Inc. 8948 Beckett Road West Chester, OH 45069 1-800-526-5224 (order line) info@diapharma.com |
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ML-00-00461REV01