A New Noggin Protein Assay

Posted: March 11, 2020

NOGGIN ELISA

A New Noggin Protein Assay:

Adding to the Toolbox for BMP and TGF-beta Research

FluoBolt™-NOGGIN

Enhanced Human Noggin Protein Detection with MEF Technology from Fianostics

NOGGIN MEF ELISA  

Figure: Noggin levels measured with a Metal-Enhanced Fluorescence format microtiter plates (MEF-MTP) vs a Standard ELISA Microtiter Plate (Standard-MTP) format with the same capture and detection antibodies.

What Makes FluoBolt™- Noggin Different?
  • Fianostics FluoBolt™ Metal Enhanced Fluorescence Immunoassays eliminate the complicated processes that come with your standard sandwich ELISA.
  • Ultra-Sensitive, Single-step Noggin Immunoassay in a standard 96-well format
  • Stable signal over time

Assay Characteristics

Method Metal Enhanced Direct Sandwich Fluorescence Immunoassay in 96-well plate format
Sample type Serum, Plasma
Standard range 0-16 ng/mL (6 standards and 2 controls in a human serum based matrix)
Conversion factor 1 pg/mL = 0.015 pmol/L (MW: 66 kD)
Sample volume 20 µl (undilited sample) / well
Incubation steps / time / temperature Single-step assay, 4 h / 37°C or overnight / RT
Sensitivity LOD (0 pmol/L + 3 SD): 1 pmol/L; LLOQ: 0.3 ng/mL
Specificity The assay detects circulating uncomplexed human Noggin
Cross reactivity Noggin is highly conserved across species. Human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin. Cross reactivity of this assay with other species than human has not been tested.

 

Visit diapharma.com for our full line of biomarker assays, including additional FluoBolt™ Assays from Fianostics.

NOGGIN MEF ELISA

Other Fianostics Assays:

  • FluoBolt™ Asporin
  • FluoBolt™ alpha-Klotho
  • FluoBolt™ Periostin

 

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NOGGIN MEF ELISA

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