Detect the Undetectable with FluoBolt™- Noggin
Posted: February 25, 2020
Noggin – A Prospective Biomarker for NAFLD Research
Serum Noggin’s utility in NASH studies
Fig. 1: Serum noggin levels (mean ± standard error of the mean) in subjects with SS, NASH, and controls. Serum noggin levels tended to be lower in SS and NASH subjects than in controls (p for trend = 0.040), without being different between SS and NASH subjects. *indicates p < 0.05 compared to the control group.
Fig. 2: Schematic representation of the proposed effects of Noggin on liver fibrosis. BMP-SMAD signaling regulates iron hemostasis, a possible mechanism of fibrosis. Noggin is an antagonist or inhibitor of BMP signaling, thus the higher the Noggin levels, the less BMP activated fibrosis.
Light up Your Results With Fianostics FluoBolt™ Technology
What Makes FluoBolt™- Noggin Different?
Fianostics FluoBolt™ Metal Enhanced Fluorescence Immunoassays eliminate the complicated processes that come with your standard sandwich ELISA.
- Ultra-Sensitive, Single-step Noggin Immunoassay in a standard 96-well format
- Stable signal over time
Enhanced Human Noggin Protein Detection with MEF technology from Fianostics
Fig. 3: Noggin levels measured with a Metal-Enhanced Fluorescence format microtiter plates (MEF-MTP) vs a Standard ELISA Microtiter Plate (Standard-MTP) format with the same capture and detection antibodies.
Assay Characteristics
| Method | Metal Enhanced Direct Sandwich Fluorescence Immunoassay in 96-well plate format |
| Sample type | Serum, Plasma |
| Standard range | 0-16 ng/mL (6 standards and 2 controls in a human serum based matrix) |
| Conversion factor | 1 pg/mL = 0.015 pmol/L (MW: 66 kD) |
| Sample volume | 20 µl (undiluted sample) / well |
| Incubation steps / time / temperature | Single-step assay, 4 h / 37°C or overnight / RT |
| Sensitivity | LOD (0 pmol/L + 3 SD): 1 pmol/L: LLOQ: 0.3 ng/mL |
| Specificity | The assay detects circulating uncomplexed human Noggin |
| Cross reactivity | Noggin is highly conserved across species. Human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin. Cross reactivity of this assay with other species than human has not been tested. |
RESEARCH USE ONLY, NOT FOR DIAGNOSTIC PURPOSES.
M30-Apoptosense® ELISA + M65® ELISA
Keratin 18 (K18/CK18) biomarkers for liver disease studies
- M30 Apoptosense® ELISA: P10011
- M30 CytoDeath™ ELISA: P10900
- M65® ELISA: P10020
- M65 EpiDeath® ELISA: P10040
RESEARCH USE ONLY, NOT FOR DIAGNOSTIC PURPOSES.
Visit diapharma.com for our full line of biomarker assays and antibodies
Questions? Comments?
Over 20 years of scientific expertise, with support just a call or click away.
Diapharma Group, Inc. 8948 Beckett Rd, West Chester, OH 45069
ML-00-00542Rev01