What substrate research methods are available?
- Chromogenix Chromogenic Substrate S-2222 Trypsin
- Chromogenix Chromogenic Substrate S-2222 Tissue Factor Pathway Inhibitor, type – I (TFPI)
- Chromogenix Chromogenic Substrate S-2238 Antithrombin
- Chromogenix Chromogenic Substrate S-2238 Antithrombin (anti-FIIa)
- Chromogenix Chromogenic Substrate S-2288 Proteolytic activity
- Chromogenix Chromogenic Substrate S-2238 Thrombin
- Chromogenix Chromogenic Substrate S-2228 Tissue Plasminogen Activator (t-PA)
- Chromogenix Chromogenic Substrate S-2251 Plasmin
- Chromogenix Chromogenic Substrate S-2302 Kallikrein
- Chromogenix Chromogenic Substrate S-2302 Kallikrein Inhibitor
- Chromogenix Chromogenic Substrate S-2302 Prekallikrein
- Chromogenix Chromogenic Substrate S-2366 Hirudin
- Chromogenix Chromogenic Substrate S-2765 Factor X
- Diapharma Chromogenic Substrate CS GK (substitute for discontinued Chromogenix S-2266) Kallikrein in urine
- Diapharma Chromogenic Substrate CS UK (substitute for discontinued Chromogenix S-2444) Urokinase
- Diapharma Chromogenic Substrate CS PSA (KLK3) (substitute for discontinued Chromogenix S-2586) Chymotrypsin
Historically, the development of a new chromogenic substrate for a specific protease has always been accompanied by the release of method sheets where the application and the methodology for a particular use were described in detail. Some methods are simple chromogenic assays where a buffer and the substrate are the only reagents to be used (i.e. proteolytic activity). Other methods instead require the use of additional compounds, which are or have been commercially available from Chromogenix or elsewhere, and consist of more reaction steps. These protocols were validated in laboratories according to the equipment and the reagents available at the time. In several cases they have been adopted in research, quality control, or routine laboratories and some of them later became Chromogenix kits now present in our product range.
During the last 20 years, the so-called Method Sheets have been taken as the starting point by several scientists, for the development of assays for particular applications, or studies. In some cases the experimental conditions have been changed according to the particular need of the investigation being done. These methods are now presented in a different form: “Research Methods”. The intention here is to provide assay protocols that are not available as kits, but complementary to our product range. In the following list, you can find the methods developed by Chromogenix for several assays. They have to be considered as general guidelines or basic tools for the development of your own assays, some of them requiring a validation within your laboratory with respect to the reagents and equipment used.
For each method, you can find an updated Bibliography with references, where the method has been used, like as originally described or with modifications. This information should facilitate and accelerate the development of the best test protocol. If you do not have that specific journal issue in your laboratory, you can visit MEDLINE. You can search for particular articles, print the abstract and order the original copy through LOANSOME DOC (and receive the document through your local library). At the same time, on our web site you have the possibility to be updated on the new products from Chromogenix.