What is the standard assay?

The software-guided pipette prompts the user to add diluent (0.3 mL, 0.9% NaCl or 0.9% NaCl/3 mM CaCl2, agonist specific), whole blood (0.3 mL), and then agonist (0.02 mL) in the nominal three-step assay.  The diluent/whole blood is incubated at 37° C for 3 minutes with stirring and aggregation, also with stirring, is measured for 6 minutes at 37° C in the standard assay for a total assay time of 9 minutes.  (Note that each test cell comes with a PTFE stir bar.)