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Posted: September 22, 2021

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Cov19 FluoBolt™-DAT Immunoassay

Quantitative Duplex Antibody Test (DAT) for antibodies to SARS-CoV-2 from Fianostics

The newest test for simultaneous, quantitative determination of anti- Spike Protein S1 Receptor-Binding Domain (anti-S1RBD) and anti-nucleocapsid (anti-NC) antibodies against SARS-CoV-2.

Cov19 FluoBolt DAT Immunoassay

MEF-FIA for SARS-CoV-2 S1RBD and NC Antibodies

Assay Benefits:

  • One measurement simultaneously determines anti-S1RBD and anti-NC Antibodies to SARS-CoV-2
  • True quantitative test (µg/ml IgG) with calibrators and controls included
  • Differentiate antibodies derived from native infection and/or vaccination
  • Patented and proven FluoBolt™ microtiter plate technology
  • Tested against NIBSC/WHO standards
  • Correlates with surrogate virus neutralization test
  • 10 µL of serum or plasma
  • Fast- 60 minute incubation

The Cov19 FluoBolt™-DAT allows the simultaneous and quantitative measurement of antibodies against the S1RBD and nucleocapsid antigen of SARS-CoV-2 within one single measurement by using only 10 µl of sample and generates results within 60 minutes.

In contrast to other antibody assays, which detect all antibodies generated against the S1- or NC-protein, the Cov19 FluoBolt™-DAT is epitope specific and detects immune-dominant antibodies species, i.e. the most important ones generated by either vaccination or infection. This is also nicely demonstrated by the fact that it detects anti-S1RBD against all dominant virus variants identified so far (see chart).

The calibrators of this assay allow for a concentration calculation of antibodies in a sample (µg/mL). Therefore, the Cov19 FluoBolt™-DAT assay may be a valuable tool for researching antibody based “protective correlate”, i.e. what amount and type of antibody gives protection for a certain period of time. The assay is also a valuable tool to differentiate unvaccinated vs. only vaccinated vs. infected samples in any SARS-CoV-2 research study.

For research use only. Not for use in diagnostic procedures.

Kit Details

SARS-CoV-2, Cov19, Covid


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