Chromogenix S-2366™ is a chromogenic substrate for factor XI and activated protein C. The substrate can be used for the determination of purified enzyme preparations as well as of Protein C and FXI in plasma.

Chemical name: L-Pyroglutamyl-L-prolyl-L-argininep-Nitroaniline hydrochloride.

Formula: < Glu-Pro-Arg-pNA·HCl

Mol. wt: 539.0

e316 nm: 1.27· 104 mol-1· L· cm-1

Solubility: > 10 mmol/L in H2O

chromogenix chromogenic substrate assay test kit

The method for the determination of activity is based on the difference in absorbance (optical density) between the pNA formed and the original substrate. The rate of pNA formation, i.e. the increase in absorbance per second at 405 nm, is proportional to the enzymatic activity and is conveniently determined with a photometer.

S-2366™ is also readily split by trypsin, thrombin, plasmin and tissue plasminogen activator. It is split by FXIIa, plasma kallikrein and FXa as well. The method for the determination of activity is based on the difference in absorbance (optical density) between the pNA formed and the original substrate. The rate of pNA formation, i.e. the increase in absorbance per second at 405 nm, is proportional to the enzymatic activity and is conveniently determined with a photometer.